Quantitative scanning differential phase contrast microscopy
نویسندگان
چکیده
منابع مشابه
Single-shot quantitative phase microscopy with color-multiplexed differential phase contrast (cDPC)
We present a new technique for quantitative phase and amplitude microscopy from a single color image with coded illumination. Our system consists of a commercial brightfield microscope with one hardware modification-an inexpensive 3D printed condenser insert. The method, color-multiplexed Differential Phase Contrast (cDPC), is a single-shot variant of Differential Phase Contrast (DPC), which re...
متن کاملQuantitative analysis of static capacitance contrast in scanning electron microscopy.
A one-dimensional model is proposed for analysing static capacitance contrast (SCC) in scanning electron microscopy. For the large-scale integrated specimen covered by an insulating thin film, the imaging signal is calculated considering the redistribution of secondary electrons (SEs) and the charging process of the equivalent effective capacitance between the irradiation point and substrate. T...
متن کاملDiffracting aperture based differential phase contrast for scanning X-ray microscopy.
It is demonstrated that in a zone plate based scanning X-ray microscope, used to image low absorbing, heterogeneous matter at a mesoscopic scale, differential phase contrast (DPC) can be implemented without adding any additional optical component to the normal scheme of the microscope. The DPC mode is simply generated by an appropriate positioning and alignment of microscope apertures. Diffract...
متن کاملScanning electron and phase-contrast microscopy of bacterial spores.
The three-dimensional immages of free and intrasporangial spores produced by scanning electron microscopy show surface structures not visible by phase-contrast microscopy. Although fine surface detail is not elucidated by scanning electron microscopy, this technique does afford a definitive picture of the general shape of spores. Spores of Bacillus popilliae, B. lentimorbus, B. thuringiensis, B...
متن کاملFirst Demonstration of Phase Contrast Scanning Transmission Electron Microscopy
Biological molecules are composed of light elements and can be considered as phase objects for the electron waves. Therefore, images of their unstained specimens show low contrast in transmission electron microscopy (TEM). Phase contrast TEM (P-TEM) is one of the powerful techniques to enhance image contrast of the phase objects [1]. In P-TEM, the phase plate (PP) is placed at a back focal plan...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Journal of Physics: Conference Series
سال: 2009
ISSN: 1742-6596
DOI: 10.1088/1742-6596/186/1/012006